Total RNA Extraction from Tardigrades

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Optimization of RNA Extraction from Rat Pancreatic Tissue

Background: Optimized RNA extraction from tissues and cell lines consists of four main stages regardless of the method of extraction: 1) homogenizing, 2) effective denaturation of proteins from RNA, 3) inactivation of ribonuclease, and 4) removal of any DNA, protein, and carbohydrate contamination. Isolation of undamaged intact RNA is challenging when the related tissue contains high levels of ...

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Tardigrades

Tardi-what? Tardigrades, also known as water bears, are a littlestudied phylum of animals. First discovered 230 years ago, there are at least 700 different species living on land, in fresh water and in the sea. The tardigrades represent a successful group of animals — in flourishing existence after about 600 million years of evolution — that could hold keys to the patterns and mechanisms by whi...

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ژورنال

عنوان ژورنال: Cold Spring Harbor Protocols

سال: 2018

ISSN: 1940-3402,1559-6095

DOI: 10.1101/pdb.prot102376